|
KMID : 0881720140290040327
|
|
Journal of Food Hygiene and Safety
2014 Volume.29 No. 4 p.327 ~ p.333
|
|
|
A Study on the Analysis of Five Artificial Sweetners in Beverages by HPLC/MS/MS
|
|
Lee Seong-Bong
Yong Kum-Chan
Hwang Sun-Il
Kim Young-Su
Jung You-Jung
Seo My-Young
Lee Chang-Hee
Sung Jin-Hee
Yoon Mi-Hye
|
|
|
Abstract
|
|
|
|
A method for analysis of five artificial sweetners (sodium saccharin, aspartame, acesulfame-K, sucralose, cyclamate) in beverage samples was developed using high-performance liquid chromatography/triple quadrupole mass spectrometry (HPLC/MS/MS). The method uses a single-step dilution for sample preperation. Seperation was achieved on a C18 column (2.1 × 150 mm, 3.5 μm) with A- 2% methanol (1 mM ammonium acetate), B-95% methanol (1 mM ammonium acetate) as mobile phase with gradient mode. The quantitation of target compounds was performed by external calibration in selected reaction monitorning (SRM) mode. The coefficient of determination of calibration curve for sodium saccharin, aspartame, acesulfame-K, sucralose and cyclamate were 0.9957, 0.9991, 0.9943, 0.9982 and 0.9948, respectively. The limits of detection (LODs) and limits of quantitation (LOQs) were in the range of 0.001~0.022 mg/L and 0.004~0.073 mg/L, repectively. Recoveries for beverage samples were in the range of 92.76~113.50% with RSD < 10.91%. The method has applied to the determination of the five sweetners in 102 beverage samples. Three artificial sweetners-aspartame, acesulfame-K, sucralose were detected from 42 samples. Sodium saccharin and cyclamate were not detected in all samples.
|
|
|
KEYWORD
|
|
|
HPLC/MS/MS, artificial sweetners, beverages
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|